Pea Fast Neutron mutants have been generated in the line JI2822 (Lewis et al. 2005) a recombinant inbred line derived from a cross between JI0015 and JI0399. JI2822 has a short stature (le), is early flowering (lfa) and has wrinkled seeds (rb) with purple spots (Fs).
Fast Neutron mutagenesis was carried out in 2001 at Oak Ridge National Laboratory (USA) using a 252Cf source with an emission rate of ca. 7.6x1010 neutrons per second. Three irradiation doses were used: 25, 20 and 15.8 Gy. Initially M2 families were developed from the 20 and 25 Gy and in 2022 the 15.8 Gy was grown to generate an additional set of M2 families.
This population was developed as a forward screening resource for gene identification (Sainsbury et al. 2006, Wang et al. 2008, Hofer et al. 2009, Hellens et al. 2010, Chen et al. 2012, Couzigou et al. 2012, Moreau et al. 2012, Zhuang et al. 2012, McAdam et al. 2017, Li et al. 2019), and then developed as a reverse genetic resource (Domoney et al. 2013). A skim sequence analysis of 250 of these FN lines has identified the genomic location of 410 deletions ranging in size from 20kb to 11Mb; deletions smaller than 20 kb could not be detected reliably by this method.