Pea Fast Neutron (FN) mutants have been generated in the recombinant inbred line JI2822, derived from a cross between JI0015 and JI0399. JI2822 is early flowering (lf) and has a short stature (le), wrinkled seeds (rb) with purple spots (Fs).

The Fast Neutron (FN) mutagenesis was carried out in 2001 at Oak Ridge National Laboratory (USA) using a 252Cf source with an emission rate of ca. 7.6x1010 neutrons per second. Three irradiation doses were used: 25, 20 and 15.8 Gy. Initially, M2 families were developed from the 20 and 25 Gy populations (Domoney et al. 2013, http://dx.doi.org/10.1071/FP13147), and in 2022, the 15.8 Gy population was grown to generate an additional set of M2 families (Ellis et al., 2026).

This FN mutagenised population was developed as a forward screening resource for gene identification (Sainsbury et al. 2006; Wang et al. 2008; Hofer et al. 2009; Hellens et al. 2010; Chen et al. 2012; Couzigou et al. 2012; Moreau et al. 2012; Zhuang et al. 2012; McAdam et al. 2017; Li et al. 2019) and then developed as a reverse genetic resource (Domoney et al. 2013).

A skim sequence data of 246 of these FN lines, examined at 10 kb windows, has identified the genomic locations of 909 unique deletions, ranging in size from 10 kb to 11 Mb. It should be noted that deletions smaller than 30 kb were not reliably detected by this method.