Background Information about Collection

This page gives Collection level information about the Collection chosen for example MTA and Cost Recovery information.

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Collection Name:Wheat Resistance gene enrichment (REN) sequencing collectionCollection Image
Collection Description:The WatRenSeq collection comprises 299 wheat landraces from the Watkins collection (Wingen et al., 2014) which were selected to represent maximum genetic diversity. In addition, 21 non-Watkins lines were chosen for other strategic purposes, e.g. cv. Paragon (which is the parent of many Watkins RIL populations), cv Fielder (transformable line), etc. The lines were subjected to resistance gene enrichment sequencing (RenSeq; Jupe et al. 2013) using a bait library designed from Triticeae resistance gene analogs and immune receptors belonging principally to the nucleotide-binding and leucine-rich repeat (NLR) class of genes and also including 275 genes conserved in grasses (Marcussen et al., 2014). The bait library was designed by Burkhard Steuernagel and Ksenia Krasileva, but has not yet been published. Self seed was obtained from the same plants that provided the DNA preps. The collection along with the RenSeq data can be used for studying wheat NLR diversity and identifying candidate resistance genes by association genetics (Arora et al., 2019).
GRU Collection Number:45
Number of Accession recorded in SeedStor:314 accessions
Collection is Publicly available:Yes
GRU Collection Type:Derived
Collection Multilateral System Status:No
Collection material is part of ITPGRFA:No
Collection Comments:

Collection Recovery and MTA templates

Signed MTA before Dispatch

EXPORT COST - Phytosanitary certification (Where needed): £186.68 Per Job
Handling Fee: £12.00 Per Job
Line Maintenance fee: £15.00 Per Line

Collection Level Links & Supplemental Data Files

Link to Internet Resource: Wingen et al., 2014
Establishing the A. E. Watkins landrace cultivar collection as a resource for systematic gene discovery in bread wheat
Link to Internet Resource: Jupe et al., 2013
Resistance gene enrichment sequencing (RenSeq) enables reannotation of the NB‐LRR gene family from sequenced plant genomes and rapid mapping of resistance loci in segregating populations
Link to Internet Resource: Marcussen et al., 2014
Ancient hybridizations among the ancestral genomes of bread wheat
Link to Internet Resource: Arora et al., 2019
Resistance gene cloning from a wild crop relative by sequence capture and association genetics